Journal: Sports Medicine - Open

Article Title: Acute Changes in Inflammatory Biomarker Levels in Recreational Runners Participating in a Marathon or Half-Marathon

doi: 10.1186/s40798-016-0045-0

Figure Lengend Snippet: Individual changes in mediators of inflammation in recreational athletes after running either a marathon or half-marathon race. * p < 0.05, ** p < 0.01, and *** p < 0.001 for comparisons between pre- and post-race values of all runners at given time points. § p < 0.05 and §§ p < 0.01 for comparisons of changes in values between full- and half-marathon runners. The highest levels of suPAR, CRP, IL-6, TNF-α, and IL-10 were noted from a marathon runner who also suffered from the most severe symptoms of post-race fatigue (case indicated by arrows ). CD163 macrophage receptor for haptoglobin-hemoglobin complexes, CRP C-reactive protein, IL interleukin, suPAR soluble urokinase-type plasminogen activator receptor, TGF-β transforming growth factor-β, TNF-α tumor necrosis factor-α, WBC white blood cells (leukocytes)

Article Snippet: The measurements of CD163 were carried out using Quantikine human CD163 ELISA assay (R&D Systems, Abingdon Science Park, UK).

Techniques:

Journal: Sports Medicine - Open

Article Title: Acute Changes in Inflammatory Biomarker Levels in Recreational Runners Participating in a Marathon or Half-Marathon

doi: 10.1186/s40798-016-0045-0

Figure Lengend Snippet: Correlations ( r ) between changes in biomarker levels, as calculated from percent change (pre to 3 h post) for each outcome variable

Article Snippet: The measurements of CD163 were carried out using Quantikine human CD163 ELISA assay (R&D Systems, Abingdon Science Park, UK).

Techniques: Biomarker Discovery

Journal: Nature Communications

Article Title: The neonatal Fc receptor (FcRn) is a pan-arterivirus receptor

doi: 10.1038/s41467-024-51142-x

Figure Lengend Snippet: A Growth of SHFV and PRRSV-2 in wild-type MA-104 cells, inoculated at MOI = 0.01 ( n = 2 biological replicates). Data shows representative results from one of two independent experiments. B Cytopathic effect (CPE) of arterivirus infection of MA-104 cells at 4 days post-inoculation, taken at ×100 magnification. Data shows representative results from one of two independent experiments. C Frequency of sgRNAs, as determined via deep sequencing of genomic DNA from MA-104 cells, prior to and after infection with arteriviruses. D Robust rank aggregation scores (RRA) scores for gene knockouts enriched in surviving MA-104 cells compared to pre-infection; top 10 hits for each screen are shown as colored datapoints. E RRA scores of SHFV vs. PRRSV-2, with CD163 , FCGRT (FcRn), and B2M circled and labeled. F Infection of cells transduced with single-guide RNA targeting grivet (for MA-104) or human (for ACHN) FCGRT (ΔFcRn), or empty vector control. Viruses tested on these cells are arranged in terms of relatedness to arteriviruses from left to right, with taxonomic relationships shown along the top. Data are presented as mean values ± SEM. Infections were performed with n = 3 biological replicates at an MOI of 0.01. Supernatant was titrated via plaque assay or focus-forming assay at the indicated time points; asterisks represent p-values (**p ≤ 0.01; *** p ≤ 0.001) derived from a two-tailed unpaired t-test with Welch correction and multiple comparisons testing. G Brightfield photographs of arterivirus infections from F taken at ×100 magnification at 3 days post-inoculation. Data shows representative results from one of two independent experiments. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies, including CD163 (goat polyclonal anti-CD163 antibody [R&D Systems, #AF1607]), FcRn (rabbit polyclonal anti-FcRn [Abcam, #ab193148]), and beta-actin (mouse monoclonal 8H10D10 [Cell Signaling Technology, #3700 S]) were diluted in TBST + 5% nonfat dried milk and incubated either overnight at 4 °C (CD163 and FcRn) or for 1 h at ambient temperature (beta-actin).

Techniques: Infection, Sequencing, Labeling, Transduction, Plasmid Preparation, Control, Plaque Assay, Focus Forming Assay, Derivative Assay, Two Tailed Test

Journal: Nature Communications

Article Title: The neonatal Fc receptor (FcRn) is a pan-arterivirus receptor

doi: 10.1038/s41467-024-51142-x

Figure Lengend Snippet: A Western blot showing gCD163 and gFcRn expression in wild-type (WT) MA-104 cells and MA-104 cells with ectopic expression of gCD163, gFcRn, or both. B Cells from A were infected with SHFV (MOI = 30) and assayed for intracellular SHFV RNA 4 h later using single molecule RNA fluorescence in situ hybridization (smRNA FISH). Confocal microscopy (×600 magnification) was used to capture 16–18 fields of view taken at random (one representative shown). Blue = nuclei; red = smFISH detecting viral (v)RNA, Scale bar = 25 μm. C Quantification of microscopy performed in B . From each field of view, the percent vRNA positive cells, average number of puncta per infected cell, and average puncta FISH intensity per infected cell were determined and graphed as independent points. Error bars represent the mean ± SEM. Data shows results from one experiment. D Time course of SHFV production from wild-type (WT) MA-104 cells (red) compared to dual-overexpressing gCD163/gFcRn cells (black) at a low (0.03, left) and high (3, right) MOI, n = 3 biological replicates. E Cytopathic effect of SHFV infection of wild-type (WT) MA-104 cells (red) compared to dual-overexpressing gCD163/gFcRn cells (black) at a low MOI (0.03) 24 hrs after inoculation. Data shows results from one representative experiment in D . F Photographs of plaque assay wells (6-well plate) fixed and stained with crystal violet 2 days after inoculation. Note the larger and more well-circumscribed plaques in the CD163/FcRn-dual-expressing cells. G Quantification of SHFV via plaque assay using MA-104 cells (WT, +gCD163, +gFcRn, or +gCD163 & +gFcRn) as substrate for infection with data presented as mean values ± SEM, n = 3 biological replicates, normalized to WT cells and analyzed using one-way ANOVA with multiple comparisons relative to WT cells: ns, not significant; ***p ≤ 0.001; ****p ≤ 0.0001. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies, including CD163 (goat polyclonal anti-CD163 antibody [R&D Systems, #AF1607]), FcRn (rabbit polyclonal anti-FcRn [Abcam, #ab193148]), and beta-actin (mouse monoclonal 8H10D10 [Cell Signaling Technology, #3700 S]) were diluted in TBST + 5% nonfat dried milk and incubated either overnight at 4 °C (CD163 and FcRn) or for 1 h at ambient temperature (beta-actin).

Techniques: Western Blot, Expressing, Infection, Fluorescence, In Situ Hybridization, Confocal Microscopy, Microscopy, Plaque Assay, Staining

Journal: Nature Communications

Article Title: The neonatal Fc receptor (FcRn) is a pan-arterivirus receptor

doi: 10.1038/s41467-024-51142-x

Figure Lengend Snippet: A FcRn orthologs from natural and potential arterivirus hosts—grivet (g), human (hu), mouse (m), pig (p), and horse (ho)–were introduced into MA-104ΔFcRn cells and inoculated with SHFV (red), PRRSV-2 (green), or EAV (blue) at an MOI of 0.01, n = 2 biological replicates. FcRn orthologs derived from the natural host of an arterivirus (i.e., positive control) are denoted by a black outline. Productive infection was then assessed via plaque assay of supernatant at 2 days post inoculation. B Similar experimental setup to A , except with CD163 orthologs in MA-104ΔCD163 cells. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies, including CD163 (goat polyclonal anti-CD163 antibody [R&D Systems, #AF1607]), FcRn (rabbit polyclonal anti-FcRn [Abcam, #ab193148]), and beta-actin (mouse monoclonal 8H10D10 [Cell Signaling Technology, #3700 S]) were diluted in TBST + 5% nonfat dried milk and incubated either overnight at 4 °C (CD163 and FcRn) or for 1 h at ambient temperature (beta-actin).

Techniques: Derivative Assay, Positive Control, Infection, Plaque Assay

Journal: iScience

Article Title: Immunometabolic adaptation in monocytes underpins functional changes during pregnancy

doi: 10.1016/j.isci.2024.109779

Figure Lengend Snippet:

Article Snippet: CD163 human antibody (clone GHI/61.1) , Miltenyi , Cat#130-123-249; RRID: AB_2819455.

Techniques: Recombinant, Adhesive, Sequencing, Modification, DC Protein Assay, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Gene Expression, Software, Saline

Journal: Respiratory Research

Article Title: Selective accumulation of langerhans-type dendritic cells in small airways of patients with COPD

doi: 10.1186/1465-9921-11-35

Figure Lengend Snippet: Characterization of langerin positive and DC-SIGN positive dendritic cels . Expression of BDCA-1, CD1a, BDCA-3, BDCA-4, CD14, CD163 and HLA-DR are represented by the blue histograms. Red histograms represent the matched isotype control staining. Results are representative of 6 independent experiments.

Article Snippet: APC conjugated anti-human CD163 was purchased at R&D systems, Abingdon, UK.

Techniques: Expressing, Control, Staining

Journal: Respiratory Research

Article Title: Selective accumulation of langerhans-type dendritic cells in small airways of patients with COPD

doi: 10.1186/1465-9921-11-35

Figure Lengend Snippet: Expression of Surface Markers on interstitial-type and langerhans-type DC (flowcytometric analysis of lung digest)

Article Snippet: APC conjugated anti-human CD163 was purchased at R&D systems, Abingdon, UK.

Techniques: Expressing, Marker, Fluorescence